11.00 Opening (Ettrich, Weiserova, Krejci)

 

 

11.10 Rudiger Ettrich (INSB, Nove Hrady): Structural aspects of translocation, binding and DNA cleavage in type I RMs, introduction into the field and state of the art

 

11. 45 Jozef Lazar (INSB, Nove Hrady): two-photon polarization microscopy in cell biology

 

12. 05 Mikalai Lapkouski (INSB, Nove Hrady): Organization of RAG1/RAG2 Signal End Cleavage complex

 

12.20 discussion

 

 

12.30 lunch (Restaurace Teresa)

 

 

14.00 Lumir Krejci (MU, Brno): DNA repair and genomic stability

 

14.05 Victoria Marini (MU, Brno): Methods part 1 (DNA substrates; EMSA; helicase, branch migration and nuclease assays, SPR technique)

 

14.20 Marek Sebesta (MU, Brno): Methods part 2 (ATPase, strand exchage, D-loop and reconstitution assays, protein modification by sumoylation)

 

14.35 Jan Vrbsky (MU, Brno): Syntetic lethal approach and generation of iPS cells.

 

14.50 discussion

 

 

15.00 Marie Weiserova (IMB, Prague): Analysis of the restriction-modification function of Type I enzymes in vivo.

 

15.15 Alena Guzanova (IMB, Prague): Biochemical analysis of the EcoR124I enzyme.

 

15:30 discussion

 

 

15.40 coffee break

 

 

16.00 Tatjana Baikova (INSB, Nove Hrady): Crystalization of EcoAi and EcoR124I mutants

 

16.10 Vitali Bialevich (INSB, Nove Hrady): Biochemical activity measurements of RM-I systems in vitro

 

16.20 David Reha (INSB, Nove Hrady): QM/MM approaches including polarization to improve ligand-binding

 

16.30 Dhiraj Sinha (INSB, Nove Hrady): Modeling helicase-endonuclease interactions

 

 

16.40 free discussion, touristic walk-around

 

 

19.00 Get-together and poster session